کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5361682 | 1388275 | 2009 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Nano-aquarium with microfluidic structures for dynamic analysis of Cryptomonas and Phormidium fabricated by femtosecond laser direct writing of photostructurable glass
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Nano-aquarium with microfluidic structures for dynamic analysis of Cryptomonas and Phormidium fabricated by femtosecond laser direct writing of photostructurable glass Nano-aquarium with microfluidic structures for dynamic analysis of Cryptomonas and Phormidium fabricated by femtosecond laser direct writing of photostructurable glass](/preview/png/5361682.png)
چکیده انگلیسی
We demonstrate fabrication of microchips with microfluidic structures for dynamic analysis of living cells using a femtosecond (fs) laser. Fs laser direct writing followed by annealing and successive wet etching in dilute hydrofluoric (HF) acid solution resulted in formation of three dimensional (3D) hollow microstructures embedded in photostructurable glass. The embedded microchannel structure enabled us to analyze unique phenomenon of Cryptomonas, which suddenly swims very fast under certain condition. Vector analysis of the driving force for the rapid motion was also carried out by introducing nano-beads into the microchannel, in which Cryptomonas was encapsulated. We also fabricated a microchip for observation of Phormidium moving toward a seedling root, which accelerates growth of the seedling. Using the embedded microchannel in the microchip, observation of Phormidium assemblage to the seedling root was easily carried out. Such microchips with microfluidic structures, referred to as a nano-aquarium, realize the efficient and highly functional observation of living cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Applied Surface Science - Volume 255, Issue 24, 30 September 2009, Pages 9893-9897
Journal: Applied Surface Science - Volume 255, Issue 24, 30 September 2009, Pages 9893-9897
نویسندگان
Yasutaka Hanada, Koji Sugioka, Hiroyuki Kawano, Ikuko Shihira Ishikawa, Atsushi Miyawaki, Katsumi Midorikawa,