کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5362827 | 1388293 | 2009 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Measurement of UV absorption of single living cell for cell manipulation using NIR femtosecond laser
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
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چکیده انگلیسی
Optical UV absorption of single human living cells ranging from 200Â nm to 360Â nm was measured in situ for the study of cell manipulation using the near-infrared (NIR) femtosecond laser. Human breast living cells of MCF-10A, MCF-7, and MDA-MB-231 were used in this experiment. The selective photo-disruptions of single living cell and its sub-organelle (nucleus) were also demonstrated using the tightly focused 790Â nm wavelength femtosecond laser with pulse duration of 110Â fs. It was found that each living cell has its own absorption spectrum in UV wavelength ranges. It was also inferred that intrinsic absorption spectrum is attributed to the amount of DNA and protein of living cell. For the study of photo-disruption of single cell using the multi-photon absorption excited by the NIR femtosecond laser pulse, the origin UV absorption spectrum of targeted living cell is important and fundamental information to understand nonlinear interaction between NIR ultrashort, high-intensity laser light and transparent living cell.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Applied Surface Science - Volume 255, Issue 9, 15 February 2009, Pages 4974-4978
Journal: Applied Surface Science - Volume 255, Issue 9, 15 February 2009, Pages 4974-4978
نویسندگان
Sung-Hak Cho, Won-Seok Chang, Kwang-Ryul Kim, Jong Wook Hong,