The biocompatibility of hydroxyapatite film deposition on micro-arc oxidation Ti6Al4V alloy

► The bioactive ceramic film was prepared by micro-arc oxidation (MAO) and hydrothermal synthesis (HS). ► Anti-hematolysis, anti-coagulation, anti-platelet adhesion and cytotoxicity tests were all used to test the biocompatibility of specimens. ► The hematolysis rate of hydroxyapatite (HA) film is only 0.994%, which cannot lead to acute hematolysis action. ► The HA modified layer shows toxicity grade 0 that means it cannot induce the cytotoxic effect.