کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5365065 1388325 2008 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Adhesion and endothelialization of endothelial cells on the surface of endovascular stents by the novel rotational culture of cells
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
Adhesion and endothelialization of endothelial cells on the surface of endovascular stents by the novel rotational culture of cells
چکیده انگلیسی

Recent researches indicate that the initial event in the implantation of endovascular stents involves mechanical injury to the vessel wall. Confluent endothelialization of vascular grafts in vitro before implantation has been suggested as a way to reduce injury of the blood vessel. The purpose of this study is to establish a useful way to improve the adhesion of endothelial cells and accelerate endothelialization on the surface of endovascular stents by a novel rotational culture device. Numerical simulation was used to predict the shear stress on the surface of stents. The number of cellular adhesion was calculated by cell counting, the cell growth was observed by scanning electron microscope and fluorescence microscope. Numerical simulation results showed that the stents was exposed to shear stress of 2.66 × 10−3 to 8.88 × 10−2 Pa. Rotational culture of human umbilical vein endothelial cells could enhance the adhesion of cells and accelerate endothelialization on the surface of stents when the culture conditions for EC adhesion were intermediate rotation speed, higher dynamic incubation times, lower cell densities.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Applied Surface Science - Volume 255, Issue 2, 15 November 2008, Pages 315-319
نویسندگان
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