کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5366548 | 1388350 | 2012 | 6 صفحه PDF | دانلود رایگان |
In this paper, an amperometric electrochemical biosensor for the detection of hydrogen peroxide (H2O2), based on gold nanoparticles (GNPs)/thionine (Thi)/GNPs/multi-walled carbon nanotubes (MWCNTs)-chitosans (Chits) composite film was developed. MWCNTs-Chits homogeneous composite was first dispersed in acetic acid solution and then the GNPs were in situ synthesized at the composite. The mixture was dripped on the glassy carbon electrode (GCE) and then the Thi was deposited by electropolymerization by Au-S or Au-N covalent bond effect and electrostatic adsorption effect as an electron transfer mediator. Finally, the mixture of GNPs and horseradish peroxidase (HRP) was assembled onto the modified electrode by covalent bond. The electrochemical behavior of the modified electrode was investigated by scanning electron microscope, cyclic voltammetry and chronoamperometry. This study introduces the in situ-synthesized GNPs on the other surface of the modified materials in H2O2 detection. The linear response range of the biosensor to H2O2 concentration was from 5 Ã 10â7 mol Lâ1 to 1.5 Ã 10â3 mol Lâ1 with a detection limit of 3.75 Ã 10â8 mol Lâ1 (based on S/N = 3).
⺠An electrochemical biosensor for the detection of hydrogen peroxide was fabricated based on the GNPs in situ synthesized. ⺠Covalent immobilization of HRP on the modified electrode shows good stability. ⺠The method would be helpful for the further fabrication of biosensors.
Journal: Applied Surface Science - Volume 258, Issue 7, 15 January 2012, Pages 2802-2807