کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5370528 1388499 2005 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of DNA chips on the molecular scale before and after hybridization with an atomic force microscope
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
Characterization of DNA chips on the molecular scale before and after hybridization with an atomic force microscope
چکیده انگلیسی

Using two different 25-mer oligonucleotide probes covalently grafted on a silicon substrate, we demonstrate how efficient atomic force microscopy (AFM) can be for monitoring each step of DNA chip preparation: from probe immobilization to hybridization on the molecular scale. We observed the probe-molecule organization on the chip after immobilization, and the target molecules, which hybridized with probes could be individually identified. This article presents a method of straightforwardly identifying not only single and double DNA strands, but also, and more significantly, the hybridized part on them.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Applied Surface Science - Volume 252, Issue 5, 15 December 2005, Pages 1765-1771
نویسندگان
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