Instrumental resolution effects in neutron scattering studies of protein dynamics

► Resolution effects in neutron scattering measurements on protein are discussed. ► Three spectrometers of differing resolution were used. ► Fitting of spectra for the determination of EISF should use a flat background. ► MSD for dry and hydrated GFP shows resolution effects for the primary onsets. ► Hydration water shows resolution effects for the onset of translational motion.