Phosphorescence quantum yield determination with time-gated fluorimeter and Tb(III)-acetylacetonate as luminescence reference

► Procedure for absolute phosphorescence quantum yield measurement is described. ► Experimental setup for absolute luminescence quantum yield standard calibration. ► Tb(acac)3 proposed as phosphorescence quantum yield reference standard. ► Luminescence quantum yield of Tb(acac)3 in cyclohexane measured. ► Luminescence lifetime of Tb(acac)3 in cyclohexane measured.