Photo-dynamics and thermal behavior of the BLUF domain containing adenylate cyclase NgPAC2 from the amoeboflagellate Naegleria gruberi NEG-M strain

► Photo-activated adenylyl cyclase NgPAC2 from Naegleria gruberi NEG-M was expressed. ► Photo-cycle dynamics of BLUF domain in NgPAC2 protein was studied. ► Photo-excitation of Flavin in signaling state caused main conversion to lumichrome. ► Concentration dependent protein aggregation and color center formation observed. ► Melting temperature and melting times at fixed temperatures were determined.