Raman spectroscopic approach to monitor the in vitro cyclization of creatine → creatinine

- In vitro monitoring of cyclization of creatine → creatinine using Raman spectroscopy.
- Time series Raman spectra of aqueous solution of creatine at pH ∼3.
- Identification of neutral, zwitterionic and protonated species of creatine in solution.
- Raman signature of ring formation of creatinine from protonated species of creatine.
- Effect of pH and temperature on in vitro cyclization process.