Studies on external electric field effects on absorption and fluorescence spectra of NADH

- Field-induced changes in optical spectra of NADH have been measured.
- The electroabsorption indicates large charge transfer in the excited state.
- The non-radiative rate is enhanced by an electric field.
- The local electric field induces the change in the fluorescence properties in cells.
- The change in the interaction between NADH and the enzyme can be analyzed by the autofluorescence.