Three-dimensional laser-scanning confocal reflecting microscope for multicolor single-molecule imaging at 1.5Â K

- 3D laser-scanning confocal single-molecule fluorescence microscope was built.
- Single-component objective of reflecting design enabled multi-color imaging at 1.5 K.
- Size of the image of single quantum dots was <1.2 times of the diffraction limit.
- 3D co-localization precision was 3 nm in x, y and 18 nm in z directions.