A possible molecular mechanism for the pressure reversal of general anaesthetics: Aggregation of halothane in POPC bilayers at high pressure

► Halothane aggregates inside POPC membranes at 200 atm but not at 400 atm. ► Experiments show that pressure reversal is observed at 80-200 atm but not at 300 atm. ► General anaesthetic aggregation probably causes pressure reversal.