Fluorescence correlation spectroscopy as a tool for measuring the rotational diffusion of macromolecules

►  Comprehensive description of fluorescence correlation measurements of the rotational diffusion of macromolecules. ► New experimental scheme for fluorescence correlation spectroscopy (FCS), called pulsed interleaved. ► Excitation FCS, for measuring all existing polarization-resolved two-photon correlations. ► Extraction of molecular shape information from FCS rotational diffusion measurements.