Influence of energy and wavelength on femtosecond laser-induced nucleation of protein

► We investigated the influence of wavelength on femtosecond laser-induced nucleation. ► The laser pulses of λ = 260 nm produced more protein dimers than that of λ = 780 nm. ► There was no difference in nucleation probability between λ = 260 nm and 780 nm. ► The nucleation could be induced beyond the threshold energy of cavitation bubbles. ► Photophysical processes such as cavitation bubble generation induce nucleation.