Terbium chelate as donor label in time-resolved fluorescence energy transfer study of DNA hybridization

►Tb-DTPA-pAS chelate can be used as an effective donor in the FRET process. ►High energy transfer efficiency was obtained between Tb-DTPA-pAS chelate and TAMRA. ►The distance between the two ends of a 23-mer double-stranded DNA was estimated. ►The donor-acceptor pair can be used as probes in the studies of DNA hybridization.