Exploring fluorescence intermittency in enzyme reactions of single lipase molecules

In this study of blinking behavior of lipase B from Candida antarctica (CALB) molecules, we consider a dynamic diffusion-controlled reactions model with two dark states and one light state in line with the well-known Michaelis-Menten enzymatic kinetics to explain the observed non-exponential blinking statistics. This model could lead to a stretched exponential decay or, t−1/2 power-law decay, depending on kinetic parameters, followed by a long-time single exponential decay.

We present a model for blinking statistics of single lipase molecules, showing either a stretched exponential decay or t−1/2 power-law decay.