Orientation determination of membrane-disruptive proteins using powder samples and rotational diffusion: A simple solid-state NMR approach

The orientation of membrane proteins undergoing uniaxial rotational diffusion can be determined without macroscopic alignment. The δ¯∥ frequency of the motionally averaged powder spectra equals the frequency of a sample aligned with the magnetic field. This allows the orientation determination of membrane-disruptive β-sheet peptides from 13CO and 15N powder spectra.