Interaction of curcumin with human serum albumin: Thermodynamic properties, fluorescence energy transfer and denaturation effects

The interaction of curcumin to human serum albumin was studied by fluorescence spectroscopy and stopped flow kinetics. Using fluorescence resonance energy transfer, the critical transfer distance between tyrptophan-214 in HSA and curcumin was calculated to be 27.44 Å. Effect of chemical and thermal denaturants on curcumin and HSA fluorescence was studied