Fluorescence resonance energy transfer between DPH and Nile Red in a lipid bilayer

(Left) FRET efficiency of the DPH-Nile Red pair in a lipid bilayer obtained from the steady state (squares) and time resolved emission (circles) properties of DPH as a function of Nile Red:lipid molar ratio. (Right) Rise of Nile Red emission due to energy transfer (625 ± 4 nm) upon selective excitation of DPH at 375 nm (b). The emission of DPH alone (a) and selective excitation of Nile Red at 450 nm (c) shows no risetime.