CEST: From basic principles to applications, challenges and opportunities

► CEST mechanism and agents: diaCEST, paraCEST, lipoCEST and hyperCEST. ► CEST comparison with off-resonance spin-lock. ► CEST-MT interaction and differentiation methods. ► Human in vivo applications of endogenous CEST: challenges and opportunities. ► Alternative detection methods: OPARACHEE, pCEST, FLEX, ZAPI, SAFARI.