Accurate determinations of one-bond 13C-13C couplings in 13C-labeled carbohydrates

▸ New constant-time method for 1-bond 13C-13C coupling determinations in 13C-labeled sugars. ▸ The method simultaneously provides active and passive 1-bond couplings. ▸ Effect of strong couplings is evaluated by computer simulations. ▸ Facilitates and improves accuracy for 13C-13C RDC determinations in overlapping resonances. ▸ The method is demonstrated in a representative collection of mono-and disaccharides.