Spectral editing for in vivo 13C magnetic resonance spectroscopy

► [1,2-13C2]acetate generates 13C-13C couplings in glutamate C5 and acetate C1. ► Glutamate C5 (182.0 ppm) and acetate C1 (182.15 ppm) are spectrally separated. ► Acetate transport and metabolism can be studied in carboxylic/amide region.