Fast detection of choline-containing metabolites in liver using 2D 1H-14N three-bond correlation (HN3BC) spectroscopy

► Choline containing metabolites in liver have been detected by a new pulse sequence HN3BC. ► This pulse sequence shows high sensitivity. ► Ex vivo carcinoma tissues can be distinguished from normal tissues in less than 3 min by using this pulse sequence. ► This method provides a new and sensitive MRS technique potentially in vivo.