Selective double quantum resolved correlation experiment for the complete separation of entire proton NMR spectra of enantiomers

The present study reports a two dimensional NMR experiment which separates single quantum spectra of enantiomers from that of a racemic mixture. This is a blend of selective double quantum refocusing, for resolving couplings and chemical shift interactions along two dimensions followed by correlation of the selectively excited protons to the entire coupled spin network. The concept is solely based on the presence of distinct intra methyl dipolar couplings of different enantiomers when dissolved in chiral orienting media. The analysis of single enantiomer spectrum obtained from respective F2 cross sections yield all the spectral information.

101Highlights► Here is an experiment for complete separation of NMR spectra of two enantiomers from their mixture. ► This is based on the J-resolved double quantum experiment. ► The separation is solely due to different RDCs for two enantiomers in chiral orienting media. ► Absence of inter molecular couplings facilitates the discrimination.