Accuracy and stability of measuring GABA, glutamate, and glutamine by proton magnetic resonance spectroscopy: A phantom study at 4Â Tesla

► J-resolved MRS is a suitable method for simultaneous quantification of glutamate, glutamine, and GABA. ► The J-resolved approach is superior to standard 30 ms PRESS in glutamine and GABA. ► Although MEGA-PRESS is the optimal approach to measuring GABA, J-resolved PRESS also provides accurate results. ► Glutamine is particularly challenging to measure in phantoms due to its lack of stability.