کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5406808 | 1506049 | 2009 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Filter-exchange PGSE NMR determination of cell membrane permeability
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Filter-exchange PGSE NMR determination of cell membrane permeability Filter-exchange PGSE NMR determination of cell membrane permeability](/preview/png/5406808.png)
چکیده انگلیسی
A new PGSE NMR sequence is introduced for measuring diffusive transport across the plasma membrane of living cells. A “diffusion filter” and a variable mixing time precedes a standard PGSE block for diffusion encoding of the NMR signal. The filter is a PGSE block optimized for selectively removing the magnetization of the extracellular water. With increasing mixing time the intra- and extracellular components approach their equilibrium fractional populations. The rate of exchange can be measured using only a few minutes of instrument time. Water exchange over the plasma membrane of starved yeast cells is studied in the temperature range +5 to +32 °C.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Magnetic Resonance - Volume 200, Issue 2, October 2009, Pages 291-295
Journal: Journal of Magnetic Resonance - Volume 200, Issue 2, October 2009, Pages 291-295
نویسندگان
Ingrid Ã
slund, Agnieszka Nowacka, Markus Nilsson, Daniel Topgaard,