کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5406840 1393191 2009 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Magnetization transfer magic-angle-spinning z-spectroscopy of excised tissues
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
Magnetization transfer magic-angle-spinning z-spectroscopy of excised tissues
چکیده انگلیسی
NMR experiments devised to aid in analyses of tissues include magnetization transfer (MT), which can highlight the signals of biological macromolecules through cross-relaxation and/or chemical exchange processes with the bulk 1H water resonance, and high-resolution magic-angle-spinning (HRMAS) methods, akin to those used in solid-state NMR to introduce additional spectral resolution via the averaging of spin anisotropies. This paper explores the result of combining these methodologies, and reports on MT “z-spectroscopy” between water and cell components in excised tissues under a variety of HRMAS conditions. Main features arising from the resulting 1H “MTMAS” experiments include strong spinning sideband manifolds centered at the liquid water shift, high-resolution isotropic features coinciding with aliphatic and amide proton resonances, and a second sideband manifold arising as spinning speeds are increased. Interpretations are given for the origin of these various features, including simulations shedding further light onto the nature of MT NMR signals observed for tissue samples. Concurrently, histological examinations are reported validating the limits of HRMAS NMR procedures to the analysis of tissue samples preserved in a number of different ways.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Magnetic Resonance - Volume 199, Issue 1, July 2009, Pages 1-9
نویسندگان
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