کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5407368 | 1393213 | 2007 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A set of BEST triple-resonance experiments for time-optimized protein resonance assignment
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A set of BEST triple-resonance experiments for time-optimized protein resonance assignment A set of BEST triple-resonance experiments for time-optimized protein resonance assignment](/preview/png/5407368.png)
چکیده انگلیسی
A series of sequential, intra-residue, and bi-directional BEST H-N-CA, H-N-CO, and H-N-CB pulse sequences is presented that extends the BEST concept introduced recently for fast multidimensional protein NMR [Schanda et al., J. Am. Chem. Soc. 128 (2006) 9042] to the complete set of experiments required for sequential resonance assignment. We demonstrate for the protein ubiquitin that 3D BEST H-N-C correlation spectra can be recorded on a 600Â MHz NMR spectrometer equipped with a cryogenic probe in only a few minutes of acquisition time with sufficient sensitivity to detect all expected cross peaks.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Magnetic Resonance - Volume 187, Issue 1, July 2007, Pages 163-169
Journal: Journal of Magnetic Resonance - Volume 187, Issue 1, July 2007, Pages 163-169
نویسندگان
Ewen Lescop, Paul Schanda, Bernhard Brutscher,