کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5407570 | 1506051 | 2006 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
HACACO revisited: Residual dipolar coupling measurements and resonance assignments in proteins
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
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چکیده انگلیسی
The revisited version of the HACACO experiment here presented, is more robust and straightforward to implement and continues to be, to a greater extent, a convenient tool for protein backbone resonance assignment. Additionally, it turns out to be a sensitive and accurate method to measure Cα-Hα residual dipolar couplings (RDCs). The performance of our new pulse scheme for measurement of RDCs was tested on two proteins with different secondary structures: one characterized by a high β-sheet content, the second dominated by the presence of α-helices. In both examples the new method provided significantly more accurate data, compared to all previously published 3D techniques.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Magnetic Resonance - Volume 180, Issue 2, June 2006, Pages 222-228
Journal: Journal of Magnetic Resonance - Volume 180, Issue 2, June 2006, Pages 222-228
نویسندگان
Daniel O. Cicero, Gian Marco Contessa, Maurizio Paci, Renzo Bazzo,