کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5407570 1506051 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
HACACO revisited: Residual dipolar coupling measurements and resonance assignments in proteins
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
HACACO revisited: Residual dipolar coupling measurements and resonance assignments in proteins
چکیده انگلیسی
The revisited version of the HACACO experiment here presented, is more robust and straightforward to implement and continues to be, to a greater extent, a convenient tool for protein backbone resonance assignment. Additionally, it turns out to be a sensitive and accurate method to measure Cα-Hα residual dipolar couplings (RDCs). The performance of our new pulse scheme for measurement of RDCs was tested on two proteins with different secondary structures: one characterized by a high β-sheet content, the second dominated by the presence of α-helices. In both examples the new method provided significantly more accurate data, compared to all previously published 3D techniques.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Magnetic Resonance - Volume 180, Issue 2, June 2006, Pages 222-228
نویسندگان
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