کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5407711 | 1393227 | 2006 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
15N spin diffusion rate in solid-state NMR of totally enriched proteins: The magic angle spinning frequency effect
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
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چکیده انگلیسی
As demonstrated by means of the one-dimensional solid-state MAS exchange experiment (CODEX), the rate of the proton driven spin diffusion between backbone 15N nuclei in totally enriched protein depends strongly on the magic angle spinning (MAS) frequency: spin diffusion at MAS frequency 16Â kHz is about 4-5 times slower as compared to that at MAS frequency 1Â kHz which is due to the averaging of the homo- and hetero-nuclear dipolar interactions by MAS. It is important that even at the highest MAS frequencies used in our experiments the spin diffusion rate is comparable or larger than typical values of the spin-lattice relaxation rates of backbone nitrogens in solid proteins. Thus, the precise quantitative analysis of 15N T1's in totally enriched solid proteins may lead to wrong quantitative results. On the other hand, the effectiveness of the 15N-15N correlation and structure determination experiments making use of 15N-15N distances can be increased by decreasing the MAS frequency as far as possible, which is counter intuitive to the commonly applied fast MAS conditions in order to reduce the dipolar-broadened line widths for increased spectral resolution.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Magnetic Resonance - Volume 182, Issue 2, October 2006, Pages 339-342
Journal: Journal of Magnetic Resonance - Volume 182, Issue 2, October 2006, Pages 339-342
نویسندگان
Alexey Krushelnitsky, Thomas Bräuniger, Detlef Reichert,