کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5435432 1509349 2017 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Water soluble axially morpholine disubstituted silicon phthalocyanines: Synthesis, characterisation, DNA/BSA binding, DNA photocleavage properties
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
پیش نمایش صفحه اول مقاله
Water soluble axially morpholine disubstituted silicon phthalocyanines: Synthesis, characterisation, DNA/BSA binding, DNA photocleavage properties
چکیده انگلیسی


- Morpholine substitue silicon phthalocyanines were synthesised.
- DNA binding study of phthalocyanines were performed by different techniques.
- The compounds interact to CT-DNA via intercalation mode of interaction.
- DNA cleavage properties of compounds were investigated by gel electrophoresis.
- Interaction of BSA with these compounds were spectroscopically examined.

In this study axially 1-morpholinopropan-2-ol disubstituted silicon phthalocyanine 4 and axially 3-morpholinophenol disubstituted silicon phthalocyanine 5 and their quaternised water soluble derivatives (4a and 5a) were synthesised for the first time. The structural characterisations of these novel compounds were performed by a combination of FT-IR, 1H NMR, UV-vis and mass. The binding propensity of the compounds with CT-DNA was performed using UV-vis absorption titration, competitive ethidium bromide and thermal denaturation experiments. These studies revealed that 4a and 5a intercalate to CT-DNA with 3.94 ± (0.11) × 104 M−1 and 1.71 ± (0.09) × 104 M−1. The DNA cleavage ability of 4a and 5a was investigated using supercoiled pBR322 plasmid DNA on agarose gel electrophoresis. 4a indicated excellent photocleavage activity under light irradiation at 650 nm in a concentration-dependent manner. However, 5a showed low cleavage activities under light irradiation at 12.5 μM and 25 μM but it had moderate cleavage activity at 50 μM. The BSA interaction experiment showed that quenching mechanisms of BSA with 4a and 5a were found as static quenching using UV-vis spectroscopy. All of these results suggest that 4a has a superior photosensitiser agent for photodynamic therapy to that of 5a due to DNA photocleavage properties.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Synthetic Metals - Volume 229, July 2017, Pages 22-32
نویسندگان
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