Antibacterial and antifungal activity of silver nanospheres synthesized by tri-sodium citrate assisted chemical approach

Antimicrobial activity of silver nanoparticles, which were synthesized by tri-sodium citrate assisted chemical approach, was evaluated. Chemical approach used for the synthesis of silver nanoparticles it was based on diminishment of silver nitrate in nearness of trisodium citrate without using any other capping and stabilizing agents. During the synthesis, four samples of silver nanoparticles were collected for comparative studies. The presence of silver nanoparticles was affirmed by analyzing the sample with X-ray diffraction, transmission electron microscopy, dynamic light scattering and ultra violet visible spectroscopy. Antimicrobial activity of some silver nanoparticle samples against two bacterial strains including Bacillus subtilis (which is Gram positive bacteria), Escherichia coli (which is Gram negative bacteria) and two fungal (yeast) strains namely Saccharomyce scerevisiae, and Candida albicans was determined. The silver nanoparticles showed the most significant inhibition activity against bacterial strains (Escherichia coli and Saccharomyces cerevisiae) in comparison to that activity towards fungal strains.