کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5503 394 2016 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Efficient bridging of 20 mm rat sciatic nerve lesions with a longitudinally micro-structured collagen scaffold
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Efficient bridging of 20 mm rat sciatic nerve lesions with a longitudinally micro-structured collagen scaffold
چکیده انگلیسی


• Collagen-based multichannel scaffold is supportive of sciatic nerve axon regeneration.
• Both sensory and motor axons regenerate through the scaffold.
• Schwann cell seeding of scaffold does not improve its regenerative properties.
• Collagen scaffold and autografts are equipotent in inducing functional recovery.

An increasing number of biomaterial nerve guides has been developed that await direct comparative testing with the ‘gold-standard’ autologous nerve graft in functional repair of peripheral nerve defects. In the present study, 20 mm rat sciatic nerve defects were bridged with either a collagen-based micro-structured nerve guide (Perimaix) or an autologous nerve graft. Axons regenerated well into the Perimaix scaffold and, the majority of these axons grew across the 20 mm defect into the distal nerve segment. In fact, both the total axon number and the number of retrogradely traced somatosensory and motor neurons extending their axons across the implant was similar between Perimaix and autologous nerve graft groups. Implantation of Schwann cell-seeded Perimaix scaffolds provided only a beneficial effect on myelination within the scaffold. Functional recovery supported by the implanted, non-seeded Perimaix scaffold was as good as that observed after the autologous nerve graft, despite the presence of thinner myelin sheaths in the Perimaix implanted nerves. These findings support the potential of the Perimaix collagen scaffold as a future off-the-shelf device for clinical applications in selected cases of traumatic peripheral nerve injury.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 75, January 2016, Pages 112–122
نویسندگان
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