کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5512672 1540438 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Nitroglycerin increases serotonin transporter expression in rat spinal cord but anandamide modulated this effect
ترجمه فارسی عنوان
نیتروگلیسیرین بیان ترانسفورماتور سروتونین را در نخاع موش نشان می دهد اما آناندامید این تاثیر را مدول می کند
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


- Administration of NTG and AEA alone are able to increase the expression of 5-HTT at the level of TNC.
- The combined nitroglycerin and anandamide treatment attenuated this effect.
- A complex interaction might be present between the serotoninergic, nitrergic and the endocannabinoid system.

Migraine is one of the most prevalent neurological diseases, which affects 16% of the total population. The exact pathomechanism of this disorder is still not well understood, but it seems that serotonin and its transporter have a crucial role in the pathogenesis.One of the animal models of migraine is the systemic administration of nitroglycerin (NTG), a nitric oxide (NO) donor. NO can initiate a central sensitization process in the trigeminal system, which is also present in migraineurs.Recent studies showed that the endocannabinoid system has a modulatory role on the trigeminal activation and sensitization.Our aim was to investigate the effect of an endogenous cannabinoid, anandamide (AEA) on the NTG-induced changes on serotonin transporter (5-HTT) expression in the upper cervical spinal cord (C1-C2) of the rat, where most of the trigeminal nociceptive afferents convey.The animals were divided into four groups. Rats in the first group, called placebo, received only the vehicle solution as treatment. In the second group, they were treated with an intraperitoneal (i.p.) injection of NTG (10 mg/kg). Rats in the third and fourth groups received i.p. AEA (2 × 5 mg/kg) half hour before and one hour after the placebo or NTG treatment. Four hours after placebo/NTG injection, the animals were perfused and the cervical spinal cords were removed for immunohistochemistry and Western blotting.Our results show that both NTG and AEA alone are able to increase 5-HTT expression in the C1-C2 segments. Combination of NTG and AEA has an opposing effect on this marker, nullifying the effects of non-combined administration, probably by negative feedback mechanisms.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chemical Neuroanatomy - Volume 85, November 2017, Pages 13-20
نویسندگان
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