کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5515 394 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Selective characterization of proteins on nanoscale concave surfaces
ترجمه فارسی عنوان
خصوصیات انتخابی پروتئین در سطوح مقعر نانو
کلمات کلیدی
نانوذرات طلا، نانوساختار نازک، جذب پروتئین، تعامل نانو و بیو
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی

Nanoscale curvature plays a critical role in nanostructure-biomolecule interactions, yet the understanding of such effects in concave nanostructures is still very limited. Because concave nanostructures usually possess convex surface curvatures as well, it is challenging to selectively study the proteins on concave surfaces alone. In this work, we have developed a novel and facile method to address this issue by desorbing proteins on the external surfaces of hollow gold nanocages (AuNG), allowing the selective characterization of retained proteins immobilized on their internal concave surfaces. The selective desorption of proteins was achieved via varying the solution ionic strength, and was demonstrated by both calculation and experimental comparison with non-hollow nanoparticles. This method has created a new platform for the discrete observation of proteins adsorbed inside AuNG hollow cores, and this work suggests an expanded biomedical application space for hollow nanomaterials.

Figure optionsDownload high-quality image (318 K)Download as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 75, January 2016, Pages 305–312
نویسندگان
, , , ,