کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5516651 1542685 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Analysis of testosterone fatty acid esters in the digestive gland of mussels by liquid chromatography-high resolution mass spectrometry
ترجمه فارسی عنوان
تجزیه و تحلیل اسید های چرب تستوسترون در غضروفی گوسفند صدف ها با استفاده از کروماتوگرافی مایع و اسپکترومتری با وضوح بالا
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


- Fatty acid esters of testosterone are synthetized in the digestive gland of mussels.
- These esters have been identified by UPLC coupled to high resolution mass spectrometry.
- The organotin compound TBT does not alter the esterification of testosterone.

Several studies have indicated that up to 70% of the total steroids detected in molluscs are in the esterified form and that pollutants, by modifying the esterification of steroids with fatty acids, might act as endocrine disrupters. However, despite the strong physiological significance of this process, there is almost no information on which fatty acids form the steroid esters and how this process is modulated. This study (a) investigates the formation of fatty acid esters of testosterone in digestive gland microsomal fractions of the mussel Mytilus galloprovincialis incubated with either palmitoly-CoA or CoA and ATP, and (b) assesses whether the endocrine disruptor tributyltin (TBT) interferes with the esterification of testosterone. Analysis of testosterone esters was performed by liquid chromatography-high resolution mass spectrometry (UPLC-HRMS). When microsomal fractions were incubated with testosterone and palmitoly-CoA, the formation of testosterone palmitate was detected. However, when microsomes were incubated with CoA and ATP, and no exogenous activated fatty acid was added, the synthesis of 16:0, 16:1, 20:5 and 22:6 testosterone esters was observed. The presence of 100 µM TBT in the incubation mixture did not significantly alter the esterification of testosterone. These results evidence the conjugation of testosterone with the most abundant fatty acids in the digestive gland microsomal fraction of mussels.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Steroids - Volume 123, July 2017, Pages 67-72
نویسندگان
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