کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5517880 | 1401037 | 2017 | 7 صفحه PDF | دانلود رایگان |
- We describe a new automated image-based method to enumerate Giardia trophozoites.
- This method permits the assessment of parasite growth at multiple time points without staining.
- The “Malaria Box” compound set was assessed for anti-Giardia activity using this approach.
- Three compounds with sub-μM activity were identified.
Giardia duodenalis is an intestinal parasite that causes giardiasis, a widespread human gastrointestinal disease. Treatment of giardiasis relies on a small arsenal of compounds that can suffer from limitations including side-effects, variable treatment efficacy and parasite drug resistance. Thus new anti-Giardia drug leads are required. The search for new compounds with anti-Giardia activity currently depends on assays that can be labour-intensive, expensive and restricted to measuring activity at a single time-point. Here we describe a new in vitro assay to assess anti-Giardia activity. This image-based assay utilizes the Perkin-Elmer Operetta® and permits automated assessment of parasite growth at multiple time points without cell-staining. Using this new approach, we assessed the “Malaria Box” compound set for anti-Giardia activity. Three compounds with sub-μM activity (IC50 0.6-0.9 μM) were identified as potential starting points for giardiasis drug discovery.
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Journal: International Journal for Parasitology: Drugs and Drug Resistance - Volume 7, Issue 1, April 2017, Pages 83-89