کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5519469 1544113 2016 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Apoptosis and gene expression in Jurkat human T cells and lymphoid tissues of fusarenon-X-treated mice
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
Apoptosis and gene expression in Jurkat human T cells and lymphoid tissues of fusarenon-X-treated mice
چکیده انگلیسی


- Fusarenon-X induced apoptosis in Jurkat human T cells.
- Fusarenon-X exhibited the up-regulation of Bax, Bid, Trp53, and Caspase-9 mRNA in Peyer's patches and thymus of mice.
- Fusarenon-X caused lymphocyte apoptosis via intrinsic apoptotic pathway.

Fusarenon X, a member of the type B trichothecene mycotoxin group, has been frequently observed, along with deoxynivalenol (DON) and nivalenol (NIV) as a contaminant in cereals. Our previous study demonstrated that a 14-day FX exposure caused apoptosis in the lymphoid tissues of mice, especially at 0.5 mg/kg bodyweight. However, the relationship between low concentrations of FX and apoptotic molecular machinery remains unclear. In the present study, we investigated the genetic regulatory mechanisms in the thymus and Peyer's patches of mice after 14 days oral administration of FX at 0.5 mg/kg bodyweight. FX caused the up-regulation of Bax, Bid, Trp53, and Caspase-9 mRNA but the relative expression of Fas, TNF, and Caspase-8 remained unchanged. Furthermore, we also determined the toxicity of FX in Jurkat T-cells. FX exhibited a concentration- and time-dependent inhibition of cell viability. Thus, incubation time and FX concentration influence the percentage of apoptotic cells. These data suggested that treatment with low dosage of FX can induce apoptosis in lymphocytes through an effect on Bax, Bid, Trp53, and Caspase-9 and therefore the mitochondrial apoptotic pathway.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicon - Volume 123, 1 December 2016, Pages 15-24
نویسندگان
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