کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5520174 1544695 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of male germ cell markers in canine testis
ترجمه فارسی عنوان
مشخصه های نشانگرهای سلول های بنیادی نر در بیضه های سگ
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
چکیده انگلیسی


- PGP9.5 and DAZL were detected in canine undifferentiated male germ cells.
- SCP3 was expressed in canine primary spermatocytes.
- C-kit was expressed stage-dependently in canine male germ and Sertoli cells.
- Acrosin was detected in C-kit-positive spematocytes and mature spermatozoa.
- Male germ cell markers present in other species are conserved in canines.

Spermatogenesis begins at puberty and continues throughout a male's life. This process is initiated and maintained by spermatogonial stem cells in the seminiferous tubules, and these cells produce haploid spermatozoa. Markers of male germ cells have been fully identified in rodents, including mice and rats, but not in canines. To characterize the canine male germ cells, histological and immunohistochemical analyses were performed, using prepubertal (1-3-month-old), early pubertal (4-month-old), and postpubertal (7-month-old) dog testes. Expression of protein gene product 9.5 (PGP9.5), deleted in azoospermia-like (DAZL), synaptonemal complex protein (SCP3), tyrosine-protein kinase Kit (C-kit), and acrosin was confirmed by immunohistochemical analysis. PGP9.5 and DAZL were detected in spermatogonia and co-localized near the basement membrane of seminiferous tubules. Some SCP3-positive cells expressed PGP9.5 but not C-kit, and most of these cells were located near the basement membrane. C-kit is a marker of differentiated spermatogenic cells. In addition, acrosin was detected in C-kit-positive spematocytes and mature spermatozoa, whereas C-kit was detected in Sertoli cells in all stages of canine testis development. We suggest that male germ cell markers detected in other species are conserved in canines. PGP9.5, DAZL, SCP3, and acrosin expressions were conserved among various species, but C-kit expression varied. This study might facilitate the identification of stage-specific canine germ cell markers and cellular mechanisms of spermatogenesis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Reproduction Science - Volume 182, July 2017, Pages 1-8
نویسندگان
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