کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5520301 | 1544694 | 2017 | 40 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Sperm membrane proteins associated with the boar semen cryopreservation
ترجمه فارسی عنوان
پروتئین های غشایی اسپرم در ارتباط با انجماد اسپرم قارچ
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
مایع منجمد، پروتئومیکس، اسپرم خوکی،
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
چکیده انگلیسی
This study aimed to define sperm membrane protein markers of semen freezability of boars with the aid of a proteomic approach. Semen from fourteen adult boars were subjected to slow freezing and rapid thawing. After thawing, sperm vigor and motility were analyzed, and based on these results, animals were separated into two groups: good (GFEs) and poor freezability (PFEs). Sperm membrane proteins were extracted and subjected to two-dimensional electrophoresis. Stained gels were analyzed by computerized resources to indicate differentially expressed protein spots, that were identified by mass spectrometry. Six animals showed good freezability with average sperm vigor and motility of 2.2 ± 0.8 and 41.8 ± 22.9, respectively, whereas eight boars showed poor freezability, with 1.9 ± 0.6 and 26.8 ± 17.5 of sperm vigor sperm motility, respectively. An average of 263 ± 62.2 spots per gel and 234.2 ± 54.6 of spots consistently present in all gels were detected. The intensities of five spots were significantly different between groups. Fc fragment of IgG binding protein and lactadherin were more intense in the PFE group, while Arylsulfatase A and F-actin capping protein subunit alpha 1 were more expressed in the GEF group. Based on their functions and interactions with other proteins, we conclude that these four sperm membrane proteins may act as potential markers of boar semen freezability.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Reproduction Science - Volume 183, August 2017, Pages 27-38
Journal: Animal Reproduction Science - Volume 183, August 2017, Pages 27-38
نویسندگان
Daianny B. Guimarães, Tatyane B. Barros, MaurÃcio F. van Tilburg, Jorge A.M. Martins, Arlindo A. Moura, Frederico B. Moreno, Ana C. Monteiro-Moreira, Renato A. Moreira, Ricardo Toniolli,