Research paperComprehensive long non-coding RNA expression profiling reveals their potential roles in systemic lupus erythematosus

- Microarray revealed abnormal lncRNA expression profile in T cells of SLE patients.
- ENST00000448942 and uc001ykl.1 were downregulated in T cells of SLE patients.
- Expressions of some lncRNAs correlate with clinical parameters of SLE patients.
- LncRNAs may operate via their correlated, relevant mRNAs or ceRNAs in SLE.

Long non-coding RNAs can regulate gene transcription, modulate protein function, and act as competing endogenous RNA. Yet, their roles in systemic lupus erythematosus remain to be elucidated. We determined the expression profiles of lncRNAs in T cells of SLE patients and healthy controls using microarrays. Up to 1935 lncRNAs and 1977 mRNAs were differentially expressed. QRT-PCR showed downregulated uc001ykl.1 and ENST00000448942 in SLE patients. Expression of uc001ykl.1 correlated with erythrocyte sedimentation rate (ESR) and C-reactive protein, whereas ENST00000448942 level correlated with ESR and anti-Sm antibodies. Short time-series expression miner analysis revealed some lncRNAs whose expressions might correlate with disease activity of SLE patients. Coding-non-coding gene coexpression analyses showed differential lncRNAs might operate via modulating expressions of their correlated, relevant mRNAs in SLE. Differential lncRNAs might also function through their ceRNAs. Our study established that the aberrant expression profiles of lncRNAs may play a role in SLE and thus warrant further investigation.