کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5531395 | 1401794 | 2016 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Biodegradable ECM-coated PCL microcarriers support scalable human early MSC expansion and in vivo bone formation
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Biodegradable ECM-coated PCL microcarriers support scalable human early MSC expansion and in vivo bone formation Biodegradable ECM-coated PCL microcarriers support scalable human early MSC expansion and in vivo bone formation](/preview/png/5531395.png)
چکیده انگلیسی
We found that heMSCs proliferate well on PCL MCs coated with a fibronectin, poly-l-lysine, and fibronectin (FN+PLL+FN) coating (cPCL MCs). During in vitro osteogenic induction, heMSCs cultured on cPCL MCs displayed a 68% increase in specific calcium deposition compared with cultures on MNL. In a mouse ectopic mineralization model, bone mass was equivalent for MNL-expanded and cPCL MC-bound heMSC implants but higher in both cases when compared with cell-free cPCL MC implants at 16 weeks post-implantation. In summary, compared with MNL cultures, biodegradable MC MSC cultures provide the benefits of large-scale expansion of cells and can be delivered in vivo, thereby eliminating the need for cell harvesting and use of scaffolds for cell delivery. These results highlight the promise of delivering heMSCs cultured on cPCL MCs for bone applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cytotherapy - Volume 18, Issue 10, October 2016, Pages 1332-1344
Journal: Cytotherapy - Volume 18, Issue 10, October 2016, Pages 1332-1344
نویسندگان
Asha Shekaran, Alan Lam, Eileen Sim, Lee Jialing, Li Jian, Jessica Toh Pei Wen, Jerry Kok Yen Chan, Mahesh Choolani, Shaul Reuveny, William Birch, Steve Oh,