کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5531694 1401808 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Short CommunicationARID3A is required for mammalian placenta development
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Short CommunicationARID3A is required for mammalian placenta development
چکیده انگلیسی


- ARID3A is highly expressed during mammalian placentation.
- Arid3a KO placentas have structural defects in placental organization.
- Arid3a KO placentas results in aberrant expression of subtype-specific placental genes.
- ARID3A loss or gain disrupts key TE-associated gene expression in human TS-like cells.

Previous studies in the mouse indicated that ARID3A plays a critical role in the first cell fate decision required for generation of trophectoderm (TE). Here, we demonstrate that ARID3A is widely expressed during mouse and human placentation and essential for early embryonic viability. ARID3A localizes to trophoblast giant cells and other trophoblast-derived cell subtypes in the junctional and labyrinth zones of the placenta. Conventional Arid3a knockout embryos suffer restricted intrauterine growth with severe defects in placental structural organization. Arid3a null placentas show aberrant expression of subtype-specific markers as well as significant alteration in cytokines, chemokines and inflammatory response-related genes, including previously established markers of human placentation disorders. BMP4-mediated induction of trophoblast stem (TS)-like cells from human induced pluripotent stem cells results in ARID3A up-regulation and cytoplasmic to nuclear translocation. Overexpression of ARID3A in BMP4-mediated TS-like cells up-regulates TE markers, whereas pluripotency markers are down-regulated. Our results reveal an essential, conserved function for ARID3A in mammalian placental development through regulation of both intrinsic and extrinsic developmental programs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental Biology - Volume 422, Issue 2, 15 February 2017, Pages 83-91
نویسندگان
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