کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5531723 1401810 2017 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The heterochronic gene Lin28 regulates amphibian metamorphosis through disturbance of thyroid hormone function
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
The heterochronic gene Lin28 regulates amphibian metamorphosis through disturbance of thyroid hormone function
چکیده انگلیسی


• Heterochronic genes are expressed during Xenopus metamorphosis.
• Overexpression of Lin28 delays Xenopus metamorphosis.
• Lin28 functions upstream or parallel to the thyroid hormone during metamorphosis.
• Lin28 regulates the expression of several components involved in TH homeostasis.

Metamorphosis is a classic example of developmental transition, which involves important morphological and physiological changes that prepare the organism for the adult life. It has been very well established that amphibian metamorphosis is mainly controlled by Thyroid Hormone (TH). Here, we show that the heterochronic gene Lin28 is downregulated during Xenopus laevis metamorphosis. Lin28 overexpression before activation of TH signaling delays metamorphosis and inhibits the expression of TH target genes. The delay in metamorphosis is rescued by incubation with exogenous TH, indicating that Lin28 works upstream or parallel to TH. High-throughput analyses performed before any delay on metamorphosis or change in TH signaling showed that overexpression of Lin28 reduces transcript levels of several hormones secreted by the pituitary, including the Thyroid-Stimulating Hormone (TSH), and regulates the expression of proteins involved in TH transport, metabolism and signaling, showing that Lin28 disrupts TH function at different levels. Our data demonstrates that the role of Lin28 in controlling developmental transitions is evolutionary conserved and establishes a functional interaction between Lin28 and thyroid hormone function introducing a new regulatory step in perinatal development with implications for our understanding of endocrine disorders.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental Biology - Volume 425, Issue 2, 15 May 2017, Pages 142–151