کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5532572 1550048 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Gene expression profiles of fin regeneration in loach (Paramisgurnus dabryanu)
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Gene expression profiles of fin regeneration in loach (Paramisgurnus dabryanu)
چکیده انگلیسی


- We demonstrated how to investigate the transcript profiles with an emphasis on bioinformatics analysis.
- Gene expression profiles during fin regeneration were studied.
- 4 valuable candidates were identified by gene screening.
- This research provided a platform for future research on pollen tube growth.

Teleost fins can regenerate accurate position-matched structure and function after amputation. However, we still lack systematic transcriptional profiling and methodologies to understand the molecular basis of fin regeneration. After histological analysis, we established a suppression subtraction hybridization library containing 418 distinct sequences expressed differentially during the process of blastema formation and differentiation in caudal fin regeneration. Genome ontology and comparative analysis of differential distribution of our data and the reference zebrafish genome showed notable subcategories, including multi-organism processes, response to stimuli, extracellular matrix, antioxidant activity, and cell junction function. KEGG pathway analysis allowed the effective identification of relevant genes in those pathways involved in tissue morphogenesis and regeneration, including tight junction, cell adhesion molecules, mTOR and Jak-STAT signaling pathway. From relevant function subcategories and signaling pathways, 78 clones were examined for further Southern-blot hybridization. Then, 17 genes were chosen and characterized using semi-quantitative PCR. Then 4 candidate genes were identified, including F11r, Mmp9, Agr2 and one without a match to any database. After real-time quantitative PCR, the results showed obvious expression changes in different periods of caudal fin regeneration. We can assume that the 4 candidates, likely valuable genes associated with fin regeneration, deserve additional attention. Thus, our study demonstrated how to investigate the transcript profiles with an emphasis on bioinformatics intervention and how to identify potential genes related to fin regeneration processes. The results also provide a foundation or knowledge for further research into genes and molecular mechanisms of fin regeneration.

After demonstrating how to investigate the transcript profiles with an emphasis on bioinformatics intervention, 4 potential genes related to fin regeneration in loach by gene screening, including F11r, Mmp9, Agr2 and one without a match to any database were identified.326

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene Expression Patterns - Volumes 25–26, November 2017, Pages 124-130
نویسندگان
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