کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5532798 1549990 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Genetic and biochemical characterization of the GH72 family of cell wall transglycosylases in Neurospora crassa
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Genetic and biochemical characterization of the GH72 family of cell wall transglycosylases in Neurospora crassa
چکیده انگلیسی


• Neurospora crassa expresses four cell wall localized GH72 transglycosylases.
• Two of the GH72 triple transglycosylase mutants, and the quadruple mutant showed dramatic cell wall defects.
• Different combinations of GH72 transglycosylases are used in different cell types during the N. crassa life cycle.
• The GH72 transglycosylases are required for the incorporation of cell wall glycoprotein into the cell wall.

The Neurospora crassa genome encodes five GH72 family transglycosylases, and four of these enzymes (GEL-1, GEL-2, GEL-3 and GEL-5) have been found to be present in the cell wall proteome. We carried out an extensive genetic analysis on the role of these four transglycosylases in cell wall biogenesis and demonstrated that the transglycosylases are required for the formation of a normal cell wall. As suggested by the proteomic analysis, we found that multiple transglycosylases were being expressed in N. crassa cells and that different combinations of the enzymes are required in different cell types. The combination of GEL-1, GEL-2 and GEL-5 is required for the growth of vegetative hyphae, while the GEL-1, GEL-2, GEL-3 combination is needed for the production of aerial hyphae and conidia. Our data demonstrates that the enzymes are redundant with partially overlapping enzymatic activities, which provides the fungus with a robust cell wall biosynthetic system. Characterization of the transglycosylase-deficient mutants demonstrated that the incorporation of cell wall proteins was severely compromised. Interestingly, we found that the transglycosylase-deficient mutant cell walls contained more β-1,3-glucan than the wild type cell wall. Our results demonstrate that the GH72 transglycosylases are not needed for the incorporation of β-1,3-glucan into the cell wall, but they are required for the incorporation of cell wall glycoprotein into the cell wall.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fungal Genetics and Biology - Volume 101, April 2017, Pages 46–54