کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5532995 1550049 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Expression profile of rrbp1 genes during embryonic development and in adult tissues of Xenopus laevis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Expression profile of rrbp1 genes during embryonic development and in adult tissues of Xenopus laevis
چکیده انگلیسی


• Expression of rrbp1b gene was firstly described in Xenopus.
• Comparison of two rrbp1 genes expression during development and in adults.
• Heart expression of rrbp1 genes was firstly detected at Stage 36.

Recent studies suggest that ribosome-binding protein 1 (RRBP1) is involved in multiple diseases such as tumorigenesis and cardiomyopathies. However, its function during embryonic development remains largely unknown. We searched Xenopus laevis database with human RRBP1 protein sequence and identified two cDNA sequences encoding Xenopus orthologs of RRBP1 including rrbp1a (NM_001089623) and rrbp1b (NM_001092468). Both genes were firstly detected at blastula stage 8 with weak signals in animal hemisphere by whole mount in situ hybridization. Evident expression of rrbp1 was mainly detected in cement gland and notochord at neurula and tailbud stages. Heart expression of rrbp1 was detected at stage 36. RT-PCR results indicated that very weak expression of rrbp1a was firstly detected in oocytes, followed by increasing expression until stage 39. Differently, very weak expression of rrbp1b was firstly observed at stage 2, and then maintained at a lower level to stage 17 followed by an intense expression from stages 19–39. Moreover, both expression profiles were also different in adult tissues. This study reports Xenopus rrbp1 expression during early embryonic development and in adult tissues. Our study will facilitate the functional analysis of Rrbp1 family during embryonic development.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene Expression Patterns - Volumes 23–24, January 2017, Pages 1–6