کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5533077 | 1402098 | 2017 | 4 صفحه PDF | دانلود رایگان |
- High content imaging-based kinome siRNA and inhibitor screens were performed.
- Aurora kinase B was the top hit for H3.3S31 phosphorylation in both screens.
- Aurora kinase B phosphorylates nucleosomal H3.3 at S31 in vitro.
Serine 31 of histone H3.3 is phosphorylated during mitosis in mammalian cells and has been shown to influence chromosome segregation. We established a screening system to identify candidate kinase(s) phosphorylating H3.3S31 by using siRNA library targeting 720 human kinases, and we confirmed the results with an independent kinase inhibitor screen. Aurora kinase B was the best candidate hit in both screens. Moreover, the kinase activity of Aurora B on H3.3S31 was biochemically confirmed with nucleosomal substrates in vitro.
Graphical Abstract78
Journal: Journal of Molecular Biology - Volume 429, Issue 13, 30 June 2017, Pages 2042-2045