کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5533814 1550566 2017 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Zebrafish akt2 is essential for survival, growth, bone development, and glucose homeostasis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Zebrafish akt2 is essential for survival, growth, bone development, and glucose homeostasis
چکیده انگلیسی


- Akt2-null zebrafish exhibit partial lethality and severe growth deficiency.
- Akt2-null zebrafish display deficiency in fin ray development.
- Akt2-null zebrafish display impaired glucose homeostasis.

As one of three akt isoforms, akt2 plays a key role in the regulation of widely divergent cellular processes in mammals. However, its role and underlying mechanisms in zebrafish remain largely unknown. To elucidate the function of akt2 in zebrafish, we generated zebrafish lacking akt2 gene via CRISPR/Cas9 technology. Akt2-null zebrafish exhibit partial lethality and severe growth deficiency, which is different from those observed in akt2-null mice. Furthermore, akt2-null zebrafish display deficiency in fin ray development, but their cartilage is not affected. Similar to observations in akt2-null mice, akt2-null zebrafish display impaired glucose homeostasis. However, in contrast to that in akt2-null mice, insulin level is lower in akt2-null zebrafish, implicating the symptoms of type I diabetes exhibited in akt2-null zebrafish. In addition, transcriptome analysis reveals that the genes involved in metabolism and osteogenesis are disturbed in akt2-null zebrafish. Taken together, these data not only support an important role of akt2 in zebrafish survival, growth, bone development and glucose homeostasis, but also suggest that akt2 has divergent functions between mice and zebrafish, even though they are evolutionarily conserved.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mechanisms of Development - Volume 143, February 2017, Pages 42-52
نویسندگان
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